KRS serum for tank. works (without preservative)
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KRS serum for bak. rabot (without preservative)
It is applied as rostostimuliruyushchy component of culture mediums at cultivation of cultures of cages of animals and viruses in conditions invitro.
Serum of blood represents native serum of blood of clinically healthy cattle, clarified, sterilized by γ - radiation method. Is issued in the glass bottles corked with rubber stoppers and rolled by metal caps of 100,0; 200,0 cm3 - for series to 10, liters; 300,0 or 400,0 cm3 - for series to 50,0 liters. Other packing coordinated in accordance with the established procedure - in canisters on 4,5, 9,0, 18,0 liters - for series from 50,0 liters is allowed.
Physical and chemical and biological indicators of serum of blood:
Appearance and color: in the frozen look firm weight from light yellow till reddish - brown color, in defrozen − opalescent liquid from light yellow till reddish - brown color. At storage of liquid loss of small flocculent deposit of the gray - white color breaking at careful stirring and ochroleucous ring on surface is allowed. In serum of blood availability of foreign impurity is not allowed. rn blood serums - 7,0 - 8,2;
Kontamination bacteria, mushrooms, mycoplasmas and viruses: no.
Biological indicators: general protein not less than 50 g/l, hemoglobin no more than 0,6 g/l, not tsitotoksichna.
Rostostimuliruyushchy properties: serum of blood of cattle as rostostimuliruyushchy component of culture mediums is used at cultivation of the intertwined cultures of cages of animals: kidneys of newborn Syrian hamster (VNK - 21, BHK - 21sl - 13); kidneys of cattle (MDBK); kidneys of embryo of pig (SPEV); primary tripsinizirovannoy cultures of chicken embryos (Federal Energy Regulatory Commission) and other adapted lines.
Serum of blood provides formation of continuous monolayer of the intertwined cultures of cages of SPEV for 3 - 5 days from the beginning of its cultivation, and provides the proliferation index (PI) not less than 5,0. Cultures of cages of MDBK formation of continuous monolayer for 4 - 5 days and SP not less than 6,0. Cultures of cages of VNK - 21 - proliferation index not less than 5,0; VNK - 21 (сl - 13) - not less than 15,0; Federal Energy Regulatory Commission - not less than 2,0.