Wednesday No. 6 GRM (for glucose fermentation definition)
In stock
Reconfirm the price with seller
- Customer pickup,
- Courier
- In detail
Description
Wednesday No. 6 GRM (for glucose fermentation definition)
Culture medium — substance or the mix of substances applied to cultivation macro - and microorganisms. There is set of standard biological culture mediums.
Ware for preparation of the environment
The ware for preparation of environments should not contain foreign matters, for example the alkalis emitted by some grades of glass, or iron oxides which can get on Wednesday at its cooking in rusty pans. It is better to use the glass, enameled or aluminum ware. Before the use the dishes need to be washed, rinsed and dried up carefully. The new glasswares is previously boiled by 30 minutes of 1 - 2% of rastvorekhlorovodorodny acid then within hour rinse in running water.
Raw materials
As initial raw materials for preparation of the majority of environments serve products of animal and plant origin, and also ready semi - finished products.
Preparation stages
- cooking: Wednesdays are cooked on naked flame, water bath, the autoclave or pulp drier digesters.
- establishment of pH: approximately make by means of indicator paper, for exact definition use potentiometer or the comparator. At sterilization of pH decreases on 0,2 therefore at first prepare more caustic solution.
- clarification is made if when cooking the environment grow turbid or darkened. For this purpose use protein of egg or serum of blood.
- filtering of the liquid and melted gelatinous environments make via wet paper filters made of cloth. Filtering of agar environments is complicated — they quickly stiffen. Usually they are filtered via the wadded and gauze filter.
- spill Wednesdays no more than on? tanks as at sterilization traffic jams can become wet and Wednesdays will lose sterility.
- sterilization: the mode of sterilization depends on structure of the environment and is specified in its recipe.
- control
- for control of sterility of the environment put for 2 days in the thermostat then they are looked through.
- chemical control finalizes pH, the content of the general and amminy nitrogen, peptone, chlorides.
- for biological control several samples of the environment sow with specially picked up cultures, and on their growth judge nutritious properties of the environment.
Similar company products
Contact the seller
Wednesday No. 6 GRM (for glucose fermentation definition)